ABOUT LABORATORY

Main directions

• Inventarization of membrane and soluble proteins of human liver cells by using biochemical analyses, 1D and/or 2D –electrophoresis, mass-spectrometry, and bioinformatics methods.
• Investigation of cytochrome P450 profile in liver microsomes with the purpose of the creation of the new diagnostic tests.
• Searching of new information about primary structure, function, mutation, substrates, inhibitors, inductors of cytochrome P450 superfamily and addition of Cytochrome P450 Knowledgebase (CPD)

Scientific collaboration

• Lab Bioinformation technology (head-Lisitsa A.V.)
• National Research Center of Surgery
• Institut of medical and biologic problems
• Bruker Daltonics GmbH

In the field :Application of Slicing of one-dimensional Gels with Subsequent Slice-by-Slice Mass-Spectrometry for the Proteomic Profiling of Human Liver Cytochromes P450.

Methods

• Isolation by ultracentrifugation of microsomal membranes;
• One and two-dimensional electrophoresis;
• Tryptic hydrolysis of proteins in SDS-PAGE gel and mass-spectrometric analysis;
• Determination the enzymic activity, using the marker substrate for different cytochromes P450 forms.

Phone: +7 (499) 255-39-60