Authors: А.V.Kharitonova¹, Е.R.Bychkov¹, O.K. Granstrem¹, U.I.Poliakov¹, A.YU.Menshikova², T.G.Evseeva², S.А.Dambinova¹

Address:

¹Institute of the Human Brain RAS 197376, St. Petersburg, Acad. Pavlova str., 9; fax (812) 234-3247, e-mail: rsn@brain.nw.ru

²Institute of Macromolecular Compounds RAS, 199004 St. Petersburg, Large ave., 31

Abstract:

Adsorption ability of few kinds of latex covered by synthetic peptide fragments of mu- and delta- opiate receptors (OR) is investigated. The levels of autoantibodies to opiate receptors fragments in the blood serum of patients with drug abuse are detected by latex agglutination and ELISA. The patients with drug abuse demonstrated positive latex agglutination reaction for level specific antibodies from 10,4 mg/ml and higher in the 71,4% of cases. The levels of autoantibodies to OR in the blood of patients with drug abuse was in 2,8 times higher of control data. The correlation between levels of autoantibodies to opiate receptors obtained by methods of latex agglutination and ELISA is revealed. The obtained data confirms our hypothesis concerning existence of specific changes in immune system linked with some CNS disorders like drug abuse. Thus, the level of autoantibodies to opiate receptors could be used as new criterion for diagnostics of opiate abuse.

Key words: autoantibodies, reaction latex agglutination, enzyme-linked immunosorbent assay

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