Authors: D.A.Kouznetsov¹, M.A.Orlova², A.G.Berdieva³, P.Z. Khasigov³

Address:

¹ Semenov Institute of Chemical Physics, RAS, Moscow, Russia

² Lomonosov Moscow State University, Russia

³ Sechenov Moscow Medical Academy Russia

Abstract:

This is a first report on the presence of creatine kinase, ATP- generating enzyme, in the venom of Vipera xanthia representing the Viperidae family of poisoning snakes. Kinetic and catalytic properties of 40 kD active monomer has been described with a following discussion on the issue of potential of the data listed for further research in either toxic coagulopathy molecular mode or enzyme application dealing with "thrombin - thrombin receptor" interaction studies. An enzyme purification procedure includes hydrophobic (phenyl-Sepharose), anion exchange (MonoQ) and affinity (ADP-Sepharose) chromatographic steps.

Key words: creatine kinase, coagulopathy, Viperidae venom.

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