Russian Journal
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Russian Journal |
ISSN 0042-8809 |
| Biomeditsinskaya Khimiya | Biomedical Chemistry |
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Issue:
Volume 50, issue 2
Title: THE ACCELERATED HYDROPHOBIC CHROMATOGRAPHY OF PLANT EXTRACTS: A PROMISING TOOL FOR study of BIOLOGICALLY ACTIVE POLYPHENOLS
Authors: A.A. Markarian1, T.D. Dargaeva2, R.N. Alyautdin1, V.L. Bourkov3
Address:
1 I.M. Sechenov Moscow Medical Academy, Moscow, Russia ( 119881, Moscow, M. Trubetskaya, 8;tel. 245-86-50, E-mail: markaria@rol.ru;
2 Russian Institute of Herbal and Aromatic Plants, Russian Academy of Agricultural Sciences, Moscow, Russia;
3 Semenov Institute for Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
Abstract:
A simple and efficient technique for fast isolation and purification of a total polyphenol fraction from dry herbal plant extract ("Phytoprost" preparation) has been developed. The method is based on sejuential cascade of 1,0-5,0 kDa ultrafiltration and the following subsejuent column chromatography of resultant oligo/polymeric compounds on Phenyl-Sepharose 4B-CL. This novel procedure allows to obtain a highly purified (not more than 0,5-0,6 % of aromatic and heterocyclic impurities) fraction of plant polyphenols in amounts required for pharmacological/biochemical screening (0,85±0,06 A280 /g dry extract) within 8-9 hours.
Key words: Polyphenols, Phenyl-Sepharose, "Phytoprost"
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