Russian Journal

ISSN 0042-8809

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Issue:
Volume 51, issue 4

Title: THE ASSAY OF TISSUE COLLAGENASE ACTIVITY USING FLUORESCEIN ISOTHIOCYANATE LABELED COLLAGEN

Authors: O. S. Ryzhakova, N. I. Solovyeva

Address: Orekhovich Institute of Biomedical Chemistry, 10 Pogodinskaya str., Moscow, 119121 Russia; Fax: 245-08057; e-mail: Nina.Solovyeva@ibmc.msk.ru

Abstract: This report describes the assay of collagenolytic activity, particularly activity of tissue collagenases, using the reconstituted fibrils of fluorescein-labeled collagen type I as substrate. Labeling of soluble rat skin collagen was carried out by the modified method of Baichi et al 1980. This method is very sensitive, reproducible and useful for screening large number of samples.

Key words: collagenolytic activity, tissue collagenase, collagen type I, fluorescein isothiocyanate

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