ÿþ<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=windows-1251"> <meta name="Author" content> <meta name="Description" content="Contents and Abstracts of Journal Voprosy Meditsinskoi Khimii (Biomedical Chemistry)."> <meta name="KeyWords" content="polyamine metabolism, inhibitors of polyamine biosynthesis, ornitine decarboxylase,polyamine oxidase"> <meta name="GENERATOR" content="Microsoft FrontPage 6.0"> <title>Russian Journal: Biomeditsinskaya Khimiya (Biomedical Chemistry) Contents &amp; Abstracts</title> </head> <body background="../../4506/grayt.gif" bgcolor="#FFFFFF" text="#000000" link="#0000FF" vlink="#800080" alink="#0000FF"> <table border="0" cellpadding="0" cellspacing="0" width="100%"> <tr> <td valign="top"> <img alt="eng1.gif (176 bytes)" border="0" src="../pic/eng2.gif" width="39" height="16"><a href="../5201r/11r.htm"><img border="0" src="../pic/rus1.gif" width="39" height="16"></a></td> <td valign="top"><p align="center"><font size="5" face="Times New Roman CE"><b>Russian Journal</b></font></td> <td align="right" valign="top"><font face="Times New Roman CE">ISSN 0042-8809</font></td> </tr> </table> <div align="center"><center> <table border="0" cellpadding="0" cellspacing="0" width="100%"> <tr> <td valign="top"><font size="5" face="Times New Roman CE"><b>Biomeditsinskaya Khimiya</b></font></td> <td valign="top"><p align="right"><font size="5" face="Times New Roman CE"><b>Biomedical Chemistry</b></font><font face="Times New Roman CE"> </font></td> </tr> </table> </center></div> <hr> <p align="center"><font face="Times New Roman CE"> <a style="font-family: Arial; font-size: 10pt; font-weight: bold; color: #003399; text-decoration: none blink" href="../../default.htm">Home</a>&nbsp; </font><font face="Times New Roman">&#1472;&nbsp; <a style="font-family: Arial; font-size: 10pt; color: #003399; font-weight: bold; text-decoration: none" href="../../board.htm">Editorial Board</a> &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../council.htm">International Advisory Board</a>&nbsp; &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../aim.htm">Aim and Scope</a> &#1472;&nbsp;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../content.htm">Contents and Abstracts</a>&nbsp; &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../information.htm">Information for Authors</a> &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../subscribe.htm">Subscribe Now</a></font></p> <hr> <p><b>Issue: <br> </b>Volume 52, issue 1</p> <p><strong> Title:</strong> DETERMINATION OF NO<sub>2<sup> </sup></sub>/ NO<sub>3<sup> </sup></sub> IN EXHALED BREATH CONDENSATE. ZINC-CATALYZED NITRATE REDUCTION </p> <p class="MsoNormal"><strong> Authors: </strong> A.S. Smirnov, A.D. Tahanovich </p> <p><b>Address: </b></p> Department of Biochemistry, Belarusian State Medical University, Minsk, Belarus; tel.:+375-296-85-75-87; e-mail: <a href="mailto:alexsmirnov@tut.by">alexsmirnov@tut.by</a> </p> <p><b>Abstract:</b> <br> Analysis of exhaled breath condensate (EBC) is a new prospective method for monitoring of inflammation and oxidative stress in lungs. The most extensively studied exhaled biomarker is nitric oxide (NO). It has very short life span and is converted into nitrites (NO<sub>2<sup> </sup></sub>) and nitrates (NO<sub>3<sup> </sup></sub>). In this study the level of NO<sub>2<sup> </sup></sub> and total level of NO<sub>2<sup> </sup></sub>/ NO<sub>3<sup> </sup></sub> have been measured in EBC of smokers and non-smokers. Prior determination of NO<sub>2<sup> </sup></sub>/ NO<sub>3<sup> </sup></sub> the nitrates were reduced to nitrites with zinc, and total level of NO<sub>2<sup> </sup></sub> was detected by Griess reaction. The mean concentrations of NO<sub>2<sup> </sup></sub> in EBC of non-smokers and smokers were 2.9±0,22 ìmol/l vs. 8,5± 1,2 ìmol/l (mean ± SD, p<0,001). The level of total nitrites NO<sub>2<sup> </sup></sub>/ NO<sub>3<sup> </sup></sub> was also higher in smokers (5,65±0,68 vs. 14,6±1,43; p<0,001). Variability index was 8%. Recovery of NO<sub>3<sup> </sup></sub> from standard solutions was 93,4±9,6%, and 91,6±8,9% from EBC. We recommend zinc reduction as good and simple method for NO<sub>3<sup> </sup></sub> determination in EBC. <p><b>Key words:</b> exhaled breath condensate, nitrate, nitrite, zinc, Griess reaction. </span></p> <b style="mso-bidi-font-weight:normal"> <hr> <p align="center"></b>[<a href="http://www.ibmc.msk.ru/pbmc/2006/5201/vol52is1.htm#11">Back</a>]<b style="mso-bidi-font-weight:normal"><b style="mso-bidi-font-weight:normal"></p> </b></b></font> </body> </html>