ÿþ<html> <head> <meta http-equiv="Content-Type" content="text/html; charset=windows-1251"> <meta name="Author" content> <meta name="Description" content="Contents and Abstracts of Journal Voprosy Meditsinskoi Khimii (Biomedical Chemistry)."> <meta name="KeyWords" content="polyamine metabolism, inhibitors of polyamine biosynthesis, ornitine decarboxylase,polyamine oxidase"> <meta name="GENERATOR" content="Microsoft FrontPage 5.0"> <title>Russian Journal: Biomeditsinskaya Khimiya (Biomedical Chemistry) Contents &amp; Abstracts</title> </head> <body background="../../4506/grayt.gif" bgcolor="#FFFFFF" text="#000000" link="#0000FF" vlink="#800080" alink="#0000FF"> <table border="0" cellpadding="0" cellspacing="0" width="100%"> <tr> <td valign="top"> <img alt="eng1.gif (176 bytes)" border="0" src="../pic/eng2.gif" width="39" height="16"><a href="../5301r/8r.htm"><img border="0" src="../pic/rus1.gif" width="39" height="16"></a></td> <td valign="top"><p align="center"><font size="5" face="Times New Roman CE"><b>Russian Journal</b></font></td> <td align="right" valign="top"><font face="Times New Roman CE">ISSN 0042-8809</font></td> </tr> </table> <div align="center"><center> <table border="0" cellpadding="0" cellspacing="0" width="100%"> <tr> <td valign="top"><font size="5" face="Times New Roman CE"><b>Biomeditsinskaya Khimiya</b></font></td> <td valign="top"><p align="right"><font size="5" face="Times New Roman CE"><b>Biomedical Chemistry</b></font><font face="Times New Roman CE"> </font></td> </tr> </table> </center></div> <hr> <p align="center"><font face="Times New Roman CE"> <a style="font-family: Arial; font-size: 10pt; font-weight: bold; color: #003399; text-decoration: none blink" href="../../default.htm">Home</a>&nbsp; </font><font face="Times New Roman">&#1472;&nbsp; <a style="font-family: Arial; font-size: 10pt; color: #003399; font-weight: bold; text-decoration: none" href="../../board.htm">Editorial Board</a> &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../council.htm">International Advisory Board</a>&nbsp; &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../aim.htm">Aim and Scope</a> &#1472;&nbsp;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../content.htm">Contents and Abstracts</a>&nbsp; &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../information.htm">Information for Authors</a> &#1472;&nbsp; <a style="text-decoration: none; color: #003399; font-family: Arial; font-size: 10pt; font-weight: bold" href="../../subscribe.htm">Subscribe Now</a></font></p> <hr> <p><b>Issue: <br> </b>Volume 53, issue 2</p> <p><strong> Title:</strong> <span lang="EN-US" style="font-family: Times New Roman; color: windowtext"> INFLUENCE OF TRYPTOPHAN CONTENT IN PROTEINS ON THE QUANTITATIVE DETERMINATION OF THIS PROTEINS USING PHOTOCHEMICAL&nbsp; </span><i> <span lang="EN-US" style="font-family: Times New Roman; color: windowtext">p</span></i><span lang="EN-US" style="font-family: Times New Roman; color: windowtext">-DIMETHYLAMINOBENZALDEHYDE</span></p> <p class="MsoNormal"><strong> Authors: </strong> <span lang="EN-US" style="font-size: 12.0pt; font-family: Times New Roman">E.A. Nikitenko, V.G. Zaitsev, O.V. Ostrovskij</span></p> <p><b>Address: </b></p> <p><span lang="EN-US" style="font-family: Times New Roman; color: windowtext"> Department of Basic and Clinical Biochemistry, Volgograd State Medical University, Volgograd, 400131 Russia; tel.: +7(8442) 38-53-63; e-mail: ol_ostr@mail.ru</span></p> <p><b>Abstract:</b> <p class="MsoNormal"> <span lang="EN-US" style="font-family: Times New Roman; color: windowtext">The interaction of proteins containing various number of Trp residues with p-dimethylamino- benzaldehyde (pDABA) was studied. A positive correlation between tryptophan content in proteins and absorbance of protein-pDABA reaction products was found (Spearman s correlation coefficient R=0,936; p&lt;0,001). Using the observed correlation we were able to predict a possible range for the absorbance of reaction products for each serum globulin and estimate the contribution of different globulins in the pDABA serum assay. We propose that the described method can be used for the determination of the globulin content in blood serum.</span></p> <p><b>Key words:</b> <span lang="EN-US" style="font-size: 12.0pt; font-family: Times New Roman"> proteins, immunoglobulins, <i>p</i>-dymethylaminobenzaldehyde, tryptophan.</span></p> <b style="mso-bidi-font-weight:normal"> <hr> <p align="center"></b>[<a href="http://www.ibmc.msk.ru/pbmc/2007/5301/vol53is1.htm#8">Back</a>]<b style="mso-bidi-font-weight:normal"><b style="mso-bidi-font-weight:normal"></p> </b></b></font> </body> </html>