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Issue:
Volume 53, issue 5

Title: SUBSTITUTED AMINOPHENOLS AND FLAVONOIDS AS POTENTIAL COMPONENTS FOR TEST-SYSTEMS OF TOTAL ANTIOXIDANT ACTIVITY.

Authors: Yu.A. Grigorenko¹, E.I. Karasyova¹, D.I. Metelitza¹, G.A. Ksendsova², V.L. Sorokin², O.I. Shadyro².

Address:
1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus’, ul. Acad. Kuprevicha, 5/2, Minsk, 220141 Belarus’; fax: (375)-(172)63-7274; е-mail: metelitza@iboch.bas-net.by;

2. Belorussian State University, Chemical Faculty, ul. Leningradskaya, 14, Minsk, 220050 Belarus’;

fax: (375)-(172)09-5464; е-mail: shadyro@open.by.

 Abstract:

A comparative kinetic study of the ortho-phenylenediamine (PDA) oxidation in the «pseudoperoxidase» system Methemalbumin-H₂O₂ in the presence of 2-amino-4-tret-butylphenol (ATBP), 2-amino-4,6-di-tret-butylphenol (ADTBP) and its four N-acylderivates, as well as flavonoids (quercetin, morin, silibin, hesperidin and naringin) has been carried out under standart conditions at 20C in the buffered physiological solution, pH 7.4, containing 5.25% ethanol and DMFA. ATBP, ADTBP and two its N-acyl-derivatives as well as all five flavonoids inhibited with different efficiency the PDA oxidation characterized in terms of the inhibition constants, K_i, M, or the percent of inhibition degree at the maximal taking concentrations of these inhibitors.

Most effective antioxidants were quercetin (K_i = 7.710^-5 M) and ATBP (K_i = 1.2610^-4 M). Using these characteristics and other necessary criteria, the pairs PDA-quercetin and PDA-ATBP were proposed for a practical application in the test-systems for total antioxidant activity of biological objects.

Key words: antioxidant activity, aminophenols, flavonoids, methemalbumin, ortho-phenylenediamine, pseudoperoxidase-catalyzed oxidation.

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