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Russian Journal |
ISSN
0042-8809 |
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Biomeditsinskaya Khimiya |
Biomedical
Chemistry |
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Issue: Volume 54, issue
2
Title:
STEROIDS,
HISTAMINE AND SEROTONIN CONSISTING OF MEDICINAL LEECH SALIVARY GLAND SECRETION
Authors: I.P.
Baskova1, Z. Ferner2, A.S. Balkina3, S.A. Kozin4, O.V.
Kharitonova1, O.V. Zavalova5, V.G. Zgoda4.
Address:
1. Biological Faculty,
M.V. Lomonosov Moscow State University, Moscow 119899, Russia; fax: (495)
939-1745; e-mail: Saliva1@yandex.ru;
2. Immuno Biological Laboratories (IBL GmbH)D-22335, Hamburg, Germany; fax: +049
40-53 28 91-11; e-mail: DFE@IBL-Hamurg.com;
3. Chemical Faculty, M.V. Lomonosov Moscow State University, Moscow 119899,
Russia; fax: (495) 939-54-17 e-mail: balkina@enzyme.chem.msu.ru;
4. Orekhovich Institute of
Biomedical Chemistry, Russian Academy of Medical Sciences,Pogodinskaya str.,
10, Moscow, 119121 Russia; fax: +7 (495) 245 08 57, e-mail: Vic@ibmh.msk.su;
5. M.M. Shemiakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry,
Russian Academy of Sciences, ul. Mikluho-Maklaya 16/10, Moscow 119997, Russia;
fax: (495)330-6538; e-mail: leech@humgen.siobc.ras.ru/
Abstract:
Lipids represent 20% of
the total weight of dried pool of medical leech salivary gland secretion (SGS)
received from about 50 individual animals. There were detected steroids, but not
phospholipids in SGS lipid fraction. Immunochemiluminescent analysis identified
free steroid hormones in SGS: cortisol, progesterone, testesterone, estradiol
and dehydroepiandrosterone. Microchromatografic-mass-spectrometric analysis of
SGS and of low-molecular weight fraction (LMW) (molecular masses ranged from 220
to 850 Da) has shown the multicomponent nature of the LMW fraction. Using
standart preparations as the reference steroid hormones (cortisol,
dehydroepiandrosterone, androstenedione and testesterone) and histamine and
serotonine have been identified in SGS.
Key words: lipids, steroid hormones, histamine, serotonin, salivary
gland secretion, medicinal leech.
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