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Issue:
Volume 54, issue 5
Title: ANTICOAGULANT ACTIVITY OF
FUCOIDANS FROM BROWN ALGAE
Authors: N.A.
Ushakova1, G.E. Morozevich1, N.E. Ustyuzhanina2,
M.I. Bilan2, A.I. Usov2, N.E. Nifantiev2,
M.E. Preobrazhenskaya1
Address:
1.
Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical
Sciences,
Pogodinskaya str., 10, Moscow, 119121 Russia; e-mail:
marina.preobrazhenskaya@ibmc.msk.ru;
2.
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Abstract:
The anticoagulant activity of polysaccharide fucoidans from 11 species of brown
algae was studied. The anticoagulant activity was measured by the activated
partial thromboplastin time (APTT), prothrombin time and thrombin time.
Inhibitory action of fucoidans
varied significantly from one species to another. Fucoidans from Laminaria
saccharina and Fucus distichus showed high anticoagulant activities,
while fucoidans from Cladosiphon okamuranus and Analipus japonicus
were almost inactive. The fucoidan inhibitory effect on thrombin and factor Xa
in the presence or in the absence of natural thrombin inhibitor, antithrombin
III (AT III), was also studied. In contrast
to the best studied anticoagulant heparin the most of the fucoidans inhibited
thrombin in the absence of AT III. In the presence of AT III inhibitory effect
of fucoidans was increased considerably. Unlike heparin, the effect of fucoidans
on factor Xa was very weak in the presence of AT III and was not observed in the
absence of AT III. The correlation between the anticoagulant activities
of this series of fucoidans and their anti-inflammatory action, studied by us
earlier, was not found. It is expected that two these types of fucoidan
activities depend on different structural features of fucoidans. These findings
show the possibility to obtain fucoidans with high anti-inflammatory action and
with low anticoagulant activity. Anticoagulant activity of the fucoidans did not
depend on the content of fucose, the other neutral sugars and sulfates in the
preparations, and also on the structure of the backbone of molecule. Taken
together, these results indicate on prospects of fucoidan study as potential
therapeutic agents.
Key words:
fucoidan,
heparin, blood coagulation, thrombin, factor Xa, antithrombin.
Биомедицинская
химия, 2008 том 54, вып. 5, с. 597-606.
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