Russian Journal

ISSN 0042-8809

Home  ׀  Editorial Board ׀  International Advisory Board  ׀  Aim and Scope ׀   Contents and Abstracts  ׀  Information for Authors ׀  Subscribe Now

Issue: Volume 55, issue 3

Title:   POTENTIATION OF NITRIC OXIDE-DEPENDЕNT ACTIVATION OF SOLUBLE GUANYLATE CYCLASE BY LAEVOMYCETIN, TETRACYCLINE AND OXOLIN

Authors: A.Yu. Shchegolev¹, T.A. Sidorova², I.S. Severina¹

Address:

¹Orechovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya ul., 10, Moscow, 119121 Russia; fax: (499)245-0857; e-mail: irina.severina@ibms.msk.ru

²Blokhin Cancer Center, Russian Academy of Medical Sciences, 24 Kashirskoye Shosse, Moscow, 115478 Russia.

    The influence of antibiotics laevomycetin and tetracycline and the antivirus agent oxolin on the activity of human platelet soluble guanylate cyclase, the stimulation of the enzyme by NO-donors (sodium nitroprusside (SNP) and spermine nanoate (spermine NONO)) and the combination of spermine NONO and YC-1 was investigated. All preparations used in the concentration range 0,1-10 мM had no effect on the basal activity of guanylate cyclase but potentiated the SNP-induced activation of this enzyme. All preparations used synergistically increased (similar to YC-1) spermine NONO-induced activation of soluble guanylate cyclase. At the same time these compounds did not produce the leftward shift of spermine NONO concentration response curve characteristic for YC-1. Moreover, all compounds used did not influence the leftward shift of spermine NONO concentration response curve obtained in the presence of YC-1. This demonstrated that there was no competition between YC-1 and the drugs for interaction with the enzyme. The revealed regulatory phenomen of laevomycetin, tetracycline and oxolin to increase synergistically NO-dependent activation of soluble guanylate cyclase may cause additional pharmacological effects during prolong treatment by these drugs. This fact is necessary taking into account.