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Russian Journal

 ISSN 0042-8809
Biomeditsinskaya Khimiya

Biomedical Chemistry

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Issue: Volume 55, issue 3

Title: MEASUREMENT OF THE ENDOGENOUS ALLOXANE IN HUMAN BLOOD

Authors: D.A. Korzhenevskiy1, A.A. Selischeva1,2, S.V. Saveliev1

Address: 

1Institute of Biomedicinal Problems, Novodanilovskaya nab., 4à, Moscow, 117105 Russia; e-mail: biomedpro@biomedpro.ru

2Department of Biology, Lomonosov Moscow State University, Vorobiovy gori, 1, Moscow, 119998 Russia

Abstract:

     To determine the endogenous alloxane content in blood of healthy donors we stabilized it by rapid lowering of pH in the sample to 2.0. Alloxane was then allowed to form a colored product in a reaction with o-phenylenediamine and its content was measured by spectrophotometry using an internal calibration curve. The experiment showed that the concentration of alloxane in blood of most of the healthy donors varied from 41 to 265 umol/l. However, a small group of healthy people was had their blood alloxane level greatly exceeding the above-mentioned values.

Key words: zinc, copper, erythrocyte, pathology.

 

Biomedical Chemistry, 2009 Volume 55, Issue 3, p. 343-349.

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