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Issue: Volume 55, issue 4

Title: COMPARATIVE CHARACTERIZATION OF CATALYTIC PROPERTIES OF MITOCHONDRIAL AND CYTOSOLIC FORMS OF NAD-DEPENDENT MALATE DEHYDROGENASE FROM RATS LIVER AT NORM AND UNDER TOXIC HEPATITIS

Authors: E.V. Mikhailova, T.N. Popova, O.A. Safonova

Address:
Voronezh State University, Biology and Soil Science Faculty, Department of Medical Biochemistry and Microbiology, University sq., 1, Voronezh, 394693 Russia; tel.: (0732) 20-82-78; fax: (0732) 20-87-55; e-mail: mikhailova@bio.vsu.ru

Abstract:

The purification and comparative characterization of some catalytic properties of liver mitochondrial and cytosolic NAD-dependent malate dehydrogenase (NAD-MDH; EC 1.1.1.37) from normal rats and rats with experimental toxic hepatitis (ETH) have been carried out. It has been found that there are some differences in catalytic and regulatory properties of liver NAD-MDH from control animals and rats with ETH. It has been shown that Fe²⁺ and Cu²⁺ ions inhibit the enzyme, and the inhibition degree is different at norm and under toxic hepatitis. Ca²⁺ ions insignificantly activate cytosolic NAD-MDH under pathology and do not influence the mitochondrial isoform.

Key words: NAD-dependent malate dehydrogenase, toxic hepatitis, properties.

Biomedical Chemistry, 2009 Volume 55, Issue 4, p. 489-499.

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