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Issue: Volume 55, issue 6

Title: ATOMIC FORCE MICROSCOPY DETECTION OF SEROLOGICAL MARKERS OF VIRAL HEPATITES B AND C

Authors: Yu.D. Ivanov¹, P.A. Frantsuzov¹, T.O. Pleshakova¹, V.S. Ziborov², S.K. Svetlov¹, N.V. Krohin¹, V.A. Konev¹, O.B. Kovalev¹, V.F. Uchaikin¹, O.N. Yastrebova³, P.G. Sveshnikov⁴, A.I. Archakov¹

Address:
¹Institute of Biomedical Chemistry RAMS 119121, Russia, Moscow, Pogodinskaya street, 10; tel.: +7 (495) 246-69-80; fax: +7 (495) 245-08-57; e-mail: Yurii.Ivanov@ibmc.msk.ru
²Scientific Association for High Temperatures RAS, Moscow
³JSV "VECTOR-BEST", Novosibirsk
⁴Russian Research Center of Molecular Diagnostics & Therapy, Moscow

Abstract:

The aim of the study was to demonstrate the possibility of detection of serological markers, containing the hepatitis B surface antigen (HBsAg) and hepatitis C virus core-antigen (HCVcoreAg) in human serum, by use of a new atomic force microscopy (AFM)-based nanotechnological approach. In this study, the immobilization on AFM-chip of antibodies against the hepatitis B virus surface antigen (anti-HBsAg) as well as the antibodies against the hepatitis C virus core antigen (anti-HCVcoreAg) was performed. It was shown that such approach enables to detect HBsAg, HCVcoreAg and the viral fragments containing these antigens in the serum. Comparative analysis of detection of HBsAg- and HCVcoreAg – containing particles by use of the AFM method vs. traditional methods (ELISA, PCR) has demonstrated the 75% coincidence of results between the AFM and the latter two methods.

Key words: atomic-force microscopy, hepatitis B virus surface antigen (HBsAg), hepatitis C virus core antigen (HCVcoreAg).

Biomedical Chemistry, 2009 Volume 55, Issue 6, p. 689-701.

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