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Issue: Volume 55, issue 6

Title: APPLICATION OF THE METHOD OF VITAL IN VIVO FLUORESCENCE INTENSITY MEASUREMENT IN BIOLOGICAL TISSUES FOR PHARMACOKINETIC STUDIES OF DIFFERENT CHLORIN-BASED PHOTOSENSITISERS

Authors: S.V. Shliakhtin¹, T.V. Trukhachova¹, G.A. Isakau¹, Y.P. Istomin²

Address:
¹RUE “Belmedpreparaty”, Fabritsyusa str. 30, Minsk, 220007 Republic of Belarus; tel./fax: +375 17 220 39 40; e-mail: S.Shlyakhtyn@rambler.ru
²National Center Of Oncology, Republic of Belarus, Minsk, Lesnoy-2

Abstract:

A precise and reproducible method of quantitative determination of the photosensitizer Photolon® in hepatic tissue of the liver of rats by means of spectrophotometric assay with preliminary chemical extraction was developed for validation of the method of in vivo measurement of a fluorescent signal of the tissue after injection of the photosensitizer. Using the ex vivo spectrophotometric technique a quantitative assessment of the results of spectrofluorescent measurements performed in vivo was made. A high correlation (R2=0,99) between results estimated by two different techniques was shown. Also validity of the simple and easy-to-use method of in vivo fluorescence intensity measurements for the investigation of the pharmacokinetics and biodistribution of different photosensitizers was demonstrated.

Key words: photodynamic therapy, Photolon®(Fotolon®), chlorin e6, pharmacokinetics.

Biomedical Chemistry, 2009 Volume 55, Issue 6, p. 766-778.

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