win.gif (204 bytes) Russian Journal ISSN 0042-8809
Voprosy Meditsinskoi Khimii

Biomedical Chemistry

Issue:
Volume 46, issue 6

Title: BACTERIAL L-ASPARAGINASES AND GLUTAMINE(ASPARAGINE)ASES: SOME PROPERTIES, STRUCTURE AND ANTITUMOUR ACTIVITY

Authors: N.N.Sokolov1, V.A.Zanin2, S.S.Alexandrova1

Address:

1Orechovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow, 119832; Tel./fax (095) 246-33-80 / (095) 245-08-57; E.mail: sokolov@medic.ibmh.msk.su

2Peoples’ Friendship University, Mikluho-Maklaya St. 6, Moscow, 117198; Tel./fax (095) 434-35-05 / (095); E.mail: zanin@med.pfu.edu.ru

Abstract:

Åxperimental material on structurally and functional organization, regulation of biosynthesis and activity, mechanism of action, genetic determinants, heterologous expression of bacterial L-asparaginases is accumulated. The modern approaches to isolation and purification of these enzymes, some questions of practical using in oncology in the schedules combined chemotherapy of leukemia the native and modified forms of L-asparaginases are discussed. The some results before carried out in the IBMC RAMS and number institutes of the Russia on study bacterial L-asparaginases and glutamine(asparagine)ases are summarized.

Key words: L-asparaginase, glutamine(asparagine)ase, enzyme therapy, leukemia, heterologous expression, Erwinia, E.coli

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