Russian Journal

ISSN 0042-8809

Issue:
Volume 46, issue 6

Title: METHODOLOGICAL ASPECTS OF THE EMPLOYMENT OF  N-(3-[2-FURYL]ACRYLOYL)-PHE-GLY-GLY    FOR PHOTOMETRIC ASSAY OF ANGIOTENSIN CONVERTING ENZYME ACTIVITY

Authors: B.Y. Altchouler

Address:  Bauman Clinical Hospital №29, Hospital sq., 2, 111020, Moscow, tel: (095) 263-06-47, fax (095) 263-26-30.

Different aspects of photometric assay of angiotensin converting enzyme (ACE) with syntetic cromogenic substrate N-(3-[2-furyl]-acryloyl)-phe-gly-gly (FAPGG) were evaluated.  At FAPGG concentration exceeding 0,025 mM the absorbance does not  follow Burger-Lambert-Bar dependences. Under there conditions the rate of absorbance   of the reaction medium strongly depends on properties of spectral weight band. This explains dramatic interamlyser variations and   complicetes standartization of the method. Linear dependence between  photometic response   and ACE activity. The employment of special inhibitor be recommended for elimination of interference.

Key words: angiotensin converting enzime, photometric, assay, method, FAPGG, activity, analysis

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