Authors: N.P.RUDKO, V.V.DAVYDOV

Address: State Medical University, Mayakowsky av., 26, Zaporozhye, 69035,Ukraine Tel. (0612) 342442, E-mail: dav@nord.vostok.net

Abstract:

This study was designed to investigate the influence of 0.01% sodium dodecyl sulphate, 1.5M and 6.0M urea and 0.03M hydrogen peroxide to the NAD(P)H: 2.6 dichlorphenolindophenol reductase activity in liver of adult and old Wistar rats during immobilizing stress. Obtained results indicate that the NADPH - dependent reductase is more resistant to modulating effect of sodium dodecyl sulphate, hydrogen peroxide and urea than NADH-dependent enzyme. The significant decrease of NADH: 2.6 dichlorphenolindophenol reductase sensitivity to the action of all studied modulators occurs in old rats. The similar changes appears in the adult rats liver during stress. The old rats immobilization is accompanied by decrease of this enzyme activity and the reduction the influence of all studied modulators to NADH: 2.6 dichlorphenolindophenol reductase as compared with adult ones. It should be suggested that revealed changes in the activity and properties of microsomal NADH: 2.6 dichlorphenolindophenol reductase promote to more decrease of the substrate hydroxylation in the liver of old rats during stress compared adult ones.

Key words: microsomal reductases, liver, stress, aging, xenobiotics metabolism.

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