Authors: T.B. Blokhina, E.A. Neshkova,   G.A.Yarovaya

Address: Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2; Moscow, 123995 Russia; fax (095)945-24-15; e-mail: acadbio@orc.ru

Abstract:

A procedure of isolation of  human blood plasma prekallikrein, coagulation factor XII and active fragment b-XIIa has been developed.  This procedure includes the traditional chromatography steps and   FPLC. Disc-electrophoresis revealed that the preparations of factor XII and b-XIIa were homogeneous. Their specific activity was 70,6 U and 2,5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities. 

 Key words: contact system activation, coagulation factor XII, fragment b-XIIa, isolation, purification, properties.

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