Russian Journal

ISSN 0042-8809

Issue:
Volume 43, issue 2

Title:
NATURALLY OCCURRING AND SEMISYNTHETIC MONOCOMPONENT FLAVOCYTOCHROMES AS CATALYTICALLY SELF-SUFFICIENT MONOOXYGENASES.

Authors:
V. V. Shumyantseva*, Yu. L. Avdeenko, T.L. Moskvitina, A.I. Archakov

Address:
Institute of Biomedical Chemistry, Pogodinskaya st. 10, 119832 Moscow, Russia, FAX 245-0857

Abstract:
In this review article the properties of cytochrome P450 BM3 (CYB 102 E.C. 1.14.14.1) and nitric oxide synthase (E.C. 1.14.13.39) have been considered. These enzymes are catalytically self-safficient P450-like systems, with flavins (FAD and FMN) and heme on one polypeptide molecule. Two- and three component cytochrome P450 systems have flavins and heme on the different protein molecules The rates of substrate metabolism catalyzed by monocomponent cytochrome P450 BM3 and nitric oxide synthase are substantially higher in comparison with two- and three-component P450 monooxygenase system. In this manuscripte authors have been discussed the properties of semisynthetic flavocytochromes based on cytochrome P450 2B4. with monooxygenase and reductase activities. These semi-artificial flavocytochromes obtained by authors serve as structural models of one-component systems and have the functional properties of two-component system (microsomal monooxygenase system).

Key words:
cytochrome P450 BM3, nitric oxide synthase, flavocytochromes, monooxygenase reactions, modelling, semisynthetic enzymes.

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