Russian Journal

ISSN 0042-8809

Issue:
Volume 43, issue 3

Title:
THE METHOD OF ESTIMATION OF LYSOZYME ACTIVITY IN SERUM WITH CHITIN AZURE AS SUBSTRATE.

Authors:
S.A.Pisarzhevsky, A.G.Globa, O.N.Shirshov, A.I.Marchuk, A.M.Svetuhin, A.A.Karelin

Address:
Vishnevsky Institute of Surgery, Russian Academy of Medcal Sciences, Bolshaya Seprukhovskaya 27, Moscow

Abstract:
The method of estimation of lysozyme activity in serum when chitin azure is used as substrate is described. The basis of incubation medium is 0,1 M acetate buffer, pH 5,0. It was judged about activity of lysozyme by fluorescence of azure in incubation medium at the end of three hour incubation at 37°C after separation of non-reacted chitin azure by short time centrifugation. It was shown that development of wound infection is accompanied by increasing of chitinase activity of lysozyme in serum.

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