Russian Journal

ISSN 0042-8809

Issue:
Volume 43, issue 4

Title:
GENE TRANSFER INTO EUCARYOTIC CELLS USING NON- CATIONIC (NEUTRAL) LIPOSOMES

Authors:
R.I.Zhdanov, O.V.Podobed, O.A.Buneeva, N.G.Kutsenco, T.A.Tsvetkova and T.P.Lavrenova

Address:
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119832, Russia

Abstract:
Transfection of plasmid DNAs containing b-galactosidase gene (pQE-LacZ) or alkaline phosphatase (pCSEAP) into L929 cell line using was studied. The complexes between plasmide DNA and liposomes containing Ca ions and glycyrrhizic acid or a- tocopherol caused successful transfection of functional genes into L929 cells. The efficiency of transfection of plasmid DNAs into L 929 cells using polynucleotide- metallo(II)-liposome complexes were 30-50% from the efficiency value of calcium phosphate coprecipitation transfection.

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